Effects of copper sulfate and Romet®-30 on gill mucous cells from channel catfish Ictalurus punctatus

Authors

  • Jaime Luis Rábago-Castro Universidad Autónoma de Tamaulipas. Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0002-0618-0490
  • Jesús Genaro Sánchez-Martínez Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas. Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0001-7410-8071
  • Jorge Loredo-Osti Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas. Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0002-6820-2974
  • Ned Iván de la Cruz-Hernández Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas. Ciudad Victoria, Tamaulipas, México.
  • Roberto Pérez-Castañeda Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas. Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0001-6602-3633

DOI:

https://doi.org/10.29059/cvpa.v1i1.6

Keywords:

cooper sulfate, histochemical, Ictalurus punctatus, mucous cells

Abstract

With the aim to study the effects of treatment with copper sulfate and Romet®-30 on the composition and number of gill mucous cells (neutral, acidic and totals), healthy channel catfish fingerlings (Ictalurus  punctatus) were exposed for eleven weeks to intermittent prophylactic doses  of  copper  sulfate  (in  baths)  or  Romet®-30  (in  feed). Each week, samples of the second gill arch were processed  for histology  and  stained  with  PAS-AB2 (periodic acid Schiff and alcian blue, pH of 2.5). The study results showed a significant increase in mucous acidic cells of fish exposed to copper, which suggest the reaction to copper sulfate.

References

Benktander, J., Sundh, H., Sundell, K., Murugan, A. V. M., Venkatakrishnan, V., Padra, J. T., Kolarevic, J., Terjesen, B. F., Gorissen, M., & Lindén, S. K. (2021). Stress impairs skin barrier function and induces α2-3 linked N-acetylneuraminic acid and core 1 O-glycans on skin mucins in Atlantic Salmon, Salmo salar. International Journal of Molecular Sciences, 22(3), 1488. https://doi.org/10.3390/ijms22031488

Cabillon, N. A. R., & Lazado, C. C. (2019). Mucosal barrier functions of fish under changing environmental conditions. Fishes, 4(1), 2. https://doi.org/10.3390/fishes4010002

Carvalho, T. L. A. B., Nascimiento, A. A., Gonçalves, C. F. S, Santos, M. A. J., & Sales A. (2020). Assessing the histological changes in fish gills as environmental biodicators in Paraty and Sepetiba bays in Rio de Janeiro, Brazil. Latin American Journal of Aquatic Research, 48(4), 590-601. http://dx.doi.org/10.3856/vol48-issue4-fulltext-2351

Dang, M., Pittman, K., Sonne, C., Hansson, S., Bach, L., Søndergaard, J., Stride, M., & Nowak, B. (2020). Histological mucous cell quantification and mucosal mapping reveal different aspects of mucous cell responses in gills and skin of shorthorn sculpins (Myoxocephalus scorpius). Fish & Shellfish Immunology, 100, 334-344. DOI: 10.1016/j.fsi.2020.03.020

De Kinkelin, P., Michel, Ch., & Ghittino, P. (1991). Tratado de las enfermedades de los peces. Editorial Acribia.

Gao, Y., Qiang, L., Zhang, L., Tan, R., Wang, X., & Yu, Y. (2023). Mucosal and humoral responses of javelin goby, Synechogobius hasta, after immersion vaccination using killed Vibrio vulnificus bacterin. Aquaculture International, 31(2), 1097-1113. https://doi.org/10.1007/s10499-022-01017-z

Haddeland, S., Lazado, C. C., Merkin, G. V., Myre, O. J., Okubamichael, M. A., Pedersen, L., & Pittman, K. (2021). Dynamic morphometrics of mucous cells reveal the minimal impact of therapeutic doses of peracetic acid on Atlantic salmon gill health. Aquaculture, 534, 736315. https://doi.org/10.1016/j.aquaculture.2020.736315

Han, F. X., Hargreves, J. A., Kingery, W. L., Huggett, D. B., & Schlenk, D. K. (2001). Accumulation, distribution and toxicity of copper in sediments of catfish ponds receiving periodic copper sulfate applications. Journal of Environmental Quality, 30(3), 912-919. https://doi.org/10.2134/jeq2001.303912x

Harris, J. E., Watson, A., & Hunt, S. (1973). Histochemical analysis of mucous cells in the epithelium of brown trout Salmo trutta L. Journal of Fish Biology, 5(3), 345-351. https://doi.org/10.1111/j.1095-8649.1973.tb04463.x

Kim, S. G., & Kang, J. C. (2004). Effect of dietary copper exposure on accumulation, growth and hematological parameters of the juvenile rockfish, Sebastes schlegeli. Marine Environmental Research, 58(1), 65-82. https://doi.org/10.1016/j.marenvres.2003.12.004

Khangarot, B. S., & Tripathi, D. M. (1991). Changes in humoral and cell-mediated responses and in skin and respiratory surfaces of catfish, Saccobranchus fossilis, following copper exposure. Ecotoxicology and Environmental Safety, 22(3), 291-308. https://doi.org/10.1016/0147-6513(91)90080-9

Lazado, C. C., Haddeland, S., Timmerhaus, G., Berg, R. S., Merkin, G., Pittman, K., & Pedersen, L. F. (2020). Morphomolecular alterations in the skin mucosa of Atlantic salmon (Salmo salar) after exposure to peracetic acid-based disinfectant. Aquaculture Reports, 17, 100368. https://doi.org/10.1016/j.aqrep.2020.100368

Marinović, Z., Miljanović, B., Urbányi, B., & Lujić, J. (2021). Gill histopathology as a biomarker for discriminating seasonal variations in water quality. Applied Sciences, 11(20), 9504. https://doi.org/10.3390/app11209504

Monteiro, S. M., Fontainhas-Fernandes, A., & Sousa, M. (2010). An immunohistochemical study of gill epithelium cells in the Nile tilapia, Oreochromis niloticus. Folia Histochemica et Cytobiologica, 48(1), 112-121. https://doi.org/10.2478/v10042-008-0105-5

Moosavi, M. J., & Shamushaki, V-A. J. (2015). Effects of different levels of copper sulfate on growth and reproductive performances in guppy (P. reticulate). Journal of Aquaculture, Research & Development, 6(2), 1-4. https://www.doi.org/10.4172/2155-9546.1000305

Mowry, R. W. (1956). Alcian blue techniques for the histochemical study of acidic carbohydrates. Journal of Histochemistry and Cytochemistry, 4, 407-408.

Nimet, J., Neves, M. P., Viana, N. P., Amorim, J. P. A., & Delariva, R. L. (2020). Histopathological alterations in gills of a fish (Astyanax bifasciatus) in neotropical streams: negative effects of riparian forest reduction and presence of pesticides. Environmental Monitoring and Assessment, 192(58). https://doi.org/10.1007/s10661-019-8030-y

Osório, J., Stiller, K. T., Reiten, B. K., Kolarevic, J., Johansen, L-H., Afonso, F. & Lazado, C. C. (2022). Intermittent administration of peracetic acid is a mild environmental stressor that elicits mucosal and systemic adaptive responses from Atlantic salmon post-smolts. BMC Zool, 7(1). https://doi.org/10.1186/s40850-021-00100-x

Ostaszewska, T., Chojnacki, M., & Kamaszewski, M. (2016). Histopathological effects of silver and copper nanoparticles on the epidermis, gills, and liver of Siberian sturgeon. Environmental Science of Pollution Research, 23(2),1621-1633. https://doi.org/10.1007/s11356-015-5391-9

Palma Leotta, M. E., Caliri, M. N., & Cáceres-Jiménez, A. R. R. (2017). Caracterización histológica e histoquímica de branquia, hígado y riñón de perca criolla (Percichthys trucha, Valenciennes, (1833) para su uso en biomonitoreo ambiental. Acta Microscopica, 26(1), 32-45

Patología en Acuicultura (2023). Hiperplasia de células mucosas branquiales en Salmón del Atlántico (Salmo salar): Histopatología. Recuperado el 10 de Mayo 2023 de https://bit.ly/41aPaLc.

Pereira, B. F., & Caetano, F. H. (2009). Histochemical technique for the detection of chloride cells in fish. Micron, 40(8), 783-786. https://doi.org/10.1016/j.micron.2009.07.010

Persson, D., Bjørgen, H., Figenschou, A., Hillestad, L., Koppang, E. O., Nødtvedt, A., &Stormoen, M. (2021). Variation in mucous cell numbers in gills of Atlantic salmon (Salmo salar) presmolt in commercial freshwater farms in Norway. Journal of Fish Diseases, 44(1), 25-32. https://doi.org/10.1111/jfd.13263

Reverter, M., Tapissier-Bontemps, N., Lecchini, D., Banaigs, B., & Sasal, P. (2018). Biological and ecological roles of external fish mucus: a review. Fishes, 3(4), 41. https://doi.org/10.3390/fishes3040041

Richardson, L. B., Burton, D. T., Block, R. M., & Stavola, A. M. (1983). Lethal and sublethal exposure and recovery effects of ozone-produced oxidants on adult white perch (Morone americana Gmelin). Water Research,17(2), 205-213. https://doi.org/10.1016/0043-1354 (83)90101-X

Rowland, S. J., Mifsud, Ch., Nixon, M., Read, P., & Landos, M. (2009). Use of formalin and copper to control icththyophthiriosis en the Australian freshwater fish silver perch (Bidyanus bidyanus Mitchell). Aquaculture Research, 40(1), 44-54. https://doi.org/10.1111/j.1365-2109.2008.02061.x

Sánchez, J. G., Speare, D. J., & Johnson, G. J. (1997). Morphometric and histochemical assessement of the branquial tissue response of rainbow trout, Onchorhynchus mykiss (Walbaum), associated with chloramine-T treatment. Journal of Fish Diseases, 20(5), 375-381. https://doi.org/10.1046/j.1365-2761.1997.00312.x

Smith, S. A., Newman, S. J., Coleman, M. P., & Alex, C. (2018). Characterization of the histologic appearance of normal gill tissue using special staining techniques. Journal of Veterinary Diagnostic Investigacion, 30(5), 688-698. https://doi.org/10.1177/1040638718791819

Tavares-Dias, M., Ferreira, J. S., Affonso, E. G., Ono, E. A., & Martins, M. L. (2011). Toxicity and effects of copper sulfate on parasitic control and hematological response of tambaqui Colossoma macropomum. Boletim do Instituto de Pesca, 37(4), 355-365.

Tavares-Dias, M. (2021). Toxic, physiological, histomorphological, growth performance and antiparasitic effects of copper sulphate in fish aquaculture. Aquaculture, 535, 736350. https://doi.org/10.1016/j.aquaculture.2021.736350

van den Heuvel, M. R., Power, M., Richards, J., Mackinnon, M., & Dixon, D. G. (2000). Disease and gill lesions in yellow perch (Perca flavescens) exposed to oil sands mining associated waters. Ecotoxicology and Environmental Safety, 46(3), 334-341. https://doi.org/10.1006/eesa.1999.1912

Published

2023-08-11

How to Cite

Rábago-Castro, J. L., Sánchez-Martínez, J. G., Loredo-Osti, J., de la Cruz-Hernández, N. I., & Pérez-Castañeda, R. (2023). Effects of copper sulfate and Romet®-30 on gill mucous cells from channel catfish Ictalurus punctatus. Ciencias Veterinarias Y Producción Animal, 1(1), 13–20. https://doi.org/10.29059/cvpa.v1i1.6

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