Pesquisa de Tritrichomonas foetus en bovinos sementales por PCR en Tamaulipas, México

Jose Carlos Mujica-García; Verónica Carvajal-de la Fuente; Raúl Sauceda-Becerra; Jorge Alva-Pérez; José Vázquez-Villanueva; Hugo Brígido Barrios García

doi:10.29059/cvpa.v2i2.31

Tritrichomonas foetus screening in breeding cattle by PCR in Tamaulipas, Mexico

Authors

Mujica-García Jose Carlos Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, México.
Verónica Carvajal-de la Fuente Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0001-6376-9771
Raúl Sauceda-Becerra Laboratorio Central Regional del Norte, Guadalupe, Nuevo León. https://orcid.org/0000-0003-0752-5339
Jorge Alva-Pérez Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0002-9320-6387
José Vázquez-Villanueva Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0001-5414-2694
Hugo Brígido Barrios García Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, México. https://orcid.org/0000-0001-7590-319X

DOI:

https://doi.org/10.29059/cvpa.v2i2.31

Keywords:

risk factors, PCR, bovine tricomonosis

Abstract

Bovine trichomoniasis is a disease capable of causing abortions in cattle. It is characterized by its asymptomatic presentation in males and is generally transmitted by venereal means. The presence of bovine trichomoniasis in a herd implies great economic losses, related to the costs of slaughtering infected males and a decrease in production. Considering that Tamaulipas is characterized by cattle production, it is important to evaluate abortive diseases such as bovine trichomoniasis, whose presence is unknown in this state, but it has been reported in neighboring states (Texas and Veracruz). The objective of this research was to determine the presence of this disease in the central area of Tamaulipas by using endpoint PCR, from samples of the preputial cavity of cattle. The samples consisted in 45 samples of beef breeds from Tamaulipas processed in a year period, all of them were negative for Tritrichomonas foetus. It is possible that the high level of reproductive technology in the area increases the probability of the presence of this parasite.

References

Aragón-López, C. E., Flores-Encinas, L. A., Pérez-Hernández, N. A., Rojas-Arzaluz, M., & Leyva Corona J. C. (2023). Desarrollo de un ensayo de amplificación isotérmica mediada por bucles con detección visual por colorimetría (cr-LAMP): una tecnología eficiente para el diagnóstico molecular a Tritrichomonas foetus en toros. https://www.ganaderia.com/destacado/desarrollo-de-un-ensayo-de-amplificacion-isotermica-mediada-por-bucles-con-deteccion-visual-por-colorimetria-cr-lamp-una-tecnologia-eficiente-para-el-diagnostico-molecular-a-tritrichomonas-foetus-en-toros

Botelho, M. P. A., Hirsch, C., Lage, A. P., Rocha, C. M. B. M. da, Dorneles, E. M. S., Cardoso, P. G., & da Costa, G. M. (2018). Prevalence of Tritrichomonas foetus and Campylobacter fetus subsp. venerealis among bulls slaughtered in the state of Minas Gerais, Brazil. Semina: Ciências Agrárias, 39(5), 2039. https://doi.org/10.5433/1679-0359.2018v39n5p2039 DOI: https://doi.org/10.5433/1679-0359.2018v39n5p2039

da Rocha, F. S., de Jesus, V. L. T., Torres, H. M., Gomes, M. J. P., de Figueiredo, M. J., do Nascimento, E. R., & de Aquino, M. H. C. (2009). Campylobacter fetus and Tritrichomonas foetus investigation in prepucial mucous of bulls from Medio Paraiba/RJ region, Brazil/Investigacao de Campylobacter fetus e Tritrichomonas foetus na mucosa prepucial de touros da regiao do Medio Paraiba, RJ. Ciencia Rural, 39(5), 1586-1590. DOI: https://doi.org/10.1590/S0103-84782009005000103

Da Silva, C., Fraga, M., Monesiglio, C., & Delpiazzo, R. (2020). Detection of Tritrichomonas foetus by PCR in preputial smegma of bulls in Uruguay. Veterinaria (Montevideo), 56(213), 17. https://doi.org/10.29155/VET.56.213.7

Dąbrowska, J., Karamon, J., Kochanowski, M., Sroka, J., Skrzypek, K., Zdybel, J., Różycki, M., Jabłoński, A., & Cencek, T. (2020). Tritrichomonas foetus: A study of prevalence in animal hosts in Poland. Pathogens, 9(3). https://doi.org/10.3390/pathogens9030203 DOI: https://doi.org/10.3390/pathogens9030203

Felleisen, R. S. (1997). Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique. Parasitology Research, 84, 153-156. https://link.springer.com/content/pdf/10.1007/s004360050374 DOI: https://doi.org/10.1007/s004360050374

Filho, R. B. D. O., Malta, K. C., Borges, J. D. M., De Oliveira, P. R. F., Filho, G. J. D. S., Nascimento, G. G., Mota, R. A., & Júnior, J. W. P. (2018). Prevalence and risk factors associated with Tritrichomonas foetus infection in cattle in the state of Paraíba, Brazil. Acta Parasitologica, 63(2), 346-353. https://doi.org/10.1515/ap-2018-0039 DOI: https://doi.org/10.1515/ap-2018-0039

Kittel, D. R., Campero, C., Van Hoosear, K. A., Rhyan, J. C., & BonDurant, R. H. (1998). Comparison of diagnostic methods for detection of active infection with Tritrichomonas foetus in beef heifers. Journal of the American Veterinary Medical Association, 213(4), 519-522. DOI: https://doi.org/10.2460/javma.1998.213.04.519

Mardones, F. O., Perez, A. M., Martínez, A., & Carpenter, T. E. (2008). Risk factors associated with Tritrichomonas foetus infection in beef herds in the Province of Buenos Aires, Argentina. Veterinary Parasitology, 153(3-4), 231-237. https://doi.org/10.1016/j.vetpar.2008.01.038 DOI: https://doi.org/10.1016/j.vetpar.2008.01.038

Martínez, C. I., Iriarte, L. S., Salas, N., Alonso, A. M., Pruzzo, C. I., dos Santos Melo, T., Pereira-Neves, A., de Miguel, N., & Coceres, V. M. (2023). Prolonged survival of venereal Tritrichomonas foetus parasite in the gastrointestinal tract, bovine fecal extract, and water. Microbiology Spectrum, 11(6). https://doi.org/10.1128/spectrum.00429-23 DOI: https://doi.org/10.1128/spectrum.00429-23

Mendoza-Ibarra, J. A., Pedraza-Díaz, S., García-Peña, F. J., Rojo-Montejo, S., Ruiz-Santa-Quiteria, J. A., San Miguel-Ibáñez, E., Navarro-Lozano, V., Ortega-Mora, L. M., Osoro, K., & Collantes-Fernandez, E. (2012). High prevalence of Tritrichomonas foetus infection in Asturiana de la Montaña beef cattle kept in extensive conditions in Northern Spain. Veterinary Journal, 193(1), 146-151. https://doi.org/10.1016/j.tvjl.2011.09.020 DOI: https://doi.org/10.1016/j.tvjl.2011.09.020

Michi, A. N., Favetto, P. H., Kastelic, J., & Cobo, E. R. (2016). A review of sexually transmitted bovine trichomoniasis and campylobacteriosis affecting cattle reproductive health. Theriogenology, 85(5), 781-791. https://doi.org/10.1016/j.theriogenology.2015.10.037 DOI: https://doi.org/10.1016/j.theriogenology.2015.10.037

Molina, L. L., Angón, E., García, A., Caballero-Villalobos, J., Giorgis, A. O., Moralejo, R. H., & Perea, J. (2018). A retrospective epidemiological analysis of shared risk factors for bovine trichomoniasis and bovine genital campylobacteriosis in La Pampa province (Argentina). Preventive Veterinary Medicine, 161, 109-114. https://doi.org/10.1016/j.prevetmed.2018.10.022 DOI: https://doi.org/10.1016/j.prevetmed.2018.10.022

Montañés, M., García-García, J. J., Benito, A., & Bodas, R. (2022). Calidad seminal e incidencia de tricomonosis y campilobacteriosis en sementales de vacuno de carne de Castilla y León. Información Técnica Económica Agraria, 119(1), 54-61. https://doi.org/10.12706/itea.2022.006 DOI: https://doi.org/10.12706/itea.2022.006

OIE. (2018). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2018). Chapter2.4.16. Trichomonosis. World Organization for Animal Health. p. 1-15. Recuperado 01/02/2023: http://www.oie.int/fileadmin/Home/fr/Health_standards/tahm/2.04.16_TRICHOMONOSIS.pdf.

Okafor, C. C., Strickland, L. G., Jones, B. M., Kania, S., Anderson, D. E., & Whitlock, B. K. (2017). Prevalence of Tritrichomonas foetus in Tennessee bulls. Veterinary Parasitology, 243, 169-175. https://doi.org/10.1016/j.vetpar.2017.06.024 DOI: https://doi.org/10.1016/j.vetpar.2017.06.024

Ondrak, J. D., Keen, J., Rupp, G., Kennedy, J., McVey, D., & Baker, W. (2010). Tritrichomonas foetus Prevention and Control in Cattle. Veterinary Clinics of North America: Food Animal Practice, 32(2), 411-423. https://doi.org/10.1016/j.cvfa.2016.01.010 DOI: https://doi.org/10.1016/j.cvfa.2016.01.010

Riegler, L. (2016). Aplicación de la Reacción en Cadena de la Polimerasa (PCR) para el diagnóstico diferencial de Tritrichomonas foetus en cultivos positivos de parásitos de la familia Trichomonadidos [Tesis de especialidad]. Universidad Nacional de la Plata.

Rojo-Montejo, S., Sanchez Sanchez, R., Ortega Mora, L. M., Osoro Otaduy, K., & Collantes-Fernandez, E. (2014). Prevalencia de la tricomonosis bovina en las razas Asturiana de la Montaña y Asturiana. http://www.serida.org/publicacionesdetalle.php?id=5723

Sambrook, J., & Russel, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed., Vol. 1). Cold Spring Harbor Laboratory Press.

Silveira, C. da S., Fraga, M., Monesiglio, C., Delpiazzo, R., Macías-Rioseco, M., Giannitti, F., & Riet-Correa, F. (2020). Detección de Tritrichomonas foetus por PCR en esmegma prepucial de toros en Uruguay. Veterinaria (Montevideo), 56(213). https://doi.org/10.29155/vet.56.213.7

Szonyi, B., Srinath, I., Schwartz, A., Clavijo, A., & Ivanek, R. (2012). Spatio-temporal epidemiology of Tritrichomonas foetus infection in Texas bulls based on state-wide diagnostic laboratory data. Veterinary Parasitology, 186(3-4), 450-455. https://doi.org/10.1016/j.vetpar.2011.11.075 DOI: https://doi.org/10.1016/j.vetpar.2011.11.075

Villalba, I., & Villalba, J. S. (2010). Raspaje prepucial de toros y colocación de tabla para protección del profesional. Sitio Argentino de Producción Animal. Repositorio Digital de Acceso Abierto. www.produccion-animal.com.ar

Villareal-Ruiz, A. A. (2020). Asociación de Tricomoniasis felina y bovina en unidades de producción pecuaria en la zona central del Estado de Veracruz [Tesis de maestría, Universidad Veracruzana]. www.uv.mx/veracruz/fmvz

Downloads

Published

2025-01-31

How to Cite

Mujica-García, J. C., Carvajal-de la Fuente, V., Sauceda-Becerra , R., Alva-Pérez, J., Vázquez-Villanueva, J., & Barrios García, H. B. (2025). Tritrichomonas foetus screening in breeding cattle by PCR in Tamaulipas, Mexico. Ciencias Veterinarias Y Producción Animal, 2(2), 18–26. https://doi.org/10.29059/cvpa.v2i2.31